中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (49): 9157-9161.doi: 10.3969/j.issn.2095-4344.2012.49.007

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

富血小板纤维蛋白诱导骨髓间充质干细胞向许旺细胞的分化

冯玉华1，董  静1，卢  蕾2，李  琦3，宋  磊4   

  1. 1吉林医药学院附属医院，吉林省吉林市 132013； 2哈励逊国际和平医院神经内科，河北省衡水市053000；3吉林大学口腔医院种植中心，吉林省长春市 130021；4吉林大学白求恩第一医院神经内科，吉林省长春市 130021
  • 收稿日期:2012-04-10 修回日期:2012-06-21 出版日期:2012-12-02 发布日期:2012-06-21
  • 作者简介:冯玉华，女，1969年生，吉林省吉林市人，汉族，2009年吉林大学毕业，主治医师，主要从事脑血管病的研究。 articlehelp@163.com

Platelet-rich fibrin induces the differentiation of bone marrow mesenchymal stem cells into
Schwann cells

Feng Yu-hua1, Dong Jing1, Lu Lei2, Li Qi3, Song Lei4   

  1. 1Affiliated Hosital of Jilin Medical College, Jinlin 132013, Jilin Province, China; 2Department of Neurology, Harrison International Peace Hospital, Hengshui 053000, Hebei Province, China; 3Implantation Centre, Stomatology Hospital, Jilin University, Changchun 130021, Jilin Province, China; 4Department of Neurology, First Hospital of Norman Bethune College of Medicine of Jilin University, Changchun 130021, Jilin Province, China 
  • Received:2012-04-10 Revised:2012-06-21 Online:2012-12-02 Published:2012-06-21
  • About author:Feng Yu-hua, Attending physician, Affiliated Hosital of Jilin Medical College, Jinlin 132013, Jilin Province, China articlehelp@163.com

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591

被引次数

6

摘要：

背景：富血小板纤维蛋白被誉为新一代血小板浓缩物，有研究表明，富血小板纤维蛋白可以诱导骨髓间充质干细胞分化为神经样细胞。

目的：观察富血小板纤维蛋白对骨髓间充质干细胞向许旺细胞分化的影响。

方法：分离兔骨髓间充质干细胞，并分为3组：对照组使用传统抗氧化剂诱导，富血小板纤维蛋白1组加入1块富血小板纤维蛋白诱导，富血小板纤维蛋白2组加入2块富血小板纤维蛋白诱导。

结果与结论：培养第3，7，14，21 天时取各组细胞行CCK-8细胞毒性检测发现，与对照组相比，富血小板纤维蛋白1组各时间点细胞增殖增快(P < 0.05)，与富血小板纤维蛋白1组相比，富血小板纤维蛋白2组各时间点细胞增殖增快(P < 0.05)；培养第21天时Real Time PCR检测发现，富血小板纤维蛋白1组细胞内S-100和神经胶质原纤维酸性蛋白mRNA表达较对照组增高(P < 0.05)，富血小板纤维蛋白1组与富血小板纤维蛋白2组相比S-100和神经胶质原纤维酸性蛋白mRNA表达差异无显著性意义(P > 0.05)；培养第21天时流式细胞术检测发现，富血小板纤维蛋白1组S-100(+)细胞百分比和神经胶质原纤维酸性蛋白(+)细胞百分比均高于对照组，富血小板纤维蛋白1组与富血小板纤维蛋白2组相比，阳性细胞百分比差异无显著性意义(P > 0.05)。结果表明，富血小板纤维蛋白可促进骨髓间充质干细胞增殖，且富血小板纤维蛋白诱导骨髓间充质干细胞分化为许旺细胞的分化率高，优于传统化学诱导方法。

关键词: 血小板纤维蛋白, 许旺细胞, 骨髓间充质干细胞, 诱导分化, 周围神经, S-100, 神经胶质原纤维酸性蛋白, 组织工程

Abstract:

BACKGROUND: Platelet-rich fibrin is known as a novel platelet condensate. Several studies have demonstrated that platelet-rich fibrin can induce the differentiation of bone marrow mesenchymal stem cells into neuronal-like cells.
OBJECTIVE: To investigate the effects of platelet-rich fibrin on differentiation of bone marrow mesenchymal stem cells into Schwann cells.
METHODS: Rabbit bone marrow mesenchymal stem cells were isolated and randomized to three groups. In the control group, conventional antioxidants were used. In the platelet-rich fibrin 1 group, one piece of platelet-rich fibrin was added to induce cell differentiation. In the platelet-rich fibrin 2 group, two pieces of platelet-rich fibrin were added to induce cell differentiation.
RESULTS AND CONCLUSION: At days 3, 7, 14, 21 days of culture, CCK-8 assay was performed to detect cell toxicity. Results showed that cell proliferation at each time point was faster in the platelet-rich fibrin 1 group than that in the control group (P < 0.05), and it was faster in the platelet-rich fibrin 2 group than that in the platelet-rich fibrin 1 group (P < 0.05). Real-time PCR results showed that at day 21 of culture, intracellular S-100 and glial fibrillary acidic protein mRNA expression levels were significantly higher in the platelet-rich fibrin 1 group than those that in the control group (P < 0.05), and no significant differences were observed between platelet-rich fibrin 1 group and platelet-rich fibrin 2 group (P > 0.05). Flow cytometry results showed that at day 21 of culture, the proportions of intracellular S-100 and glial fibrillary acidic protein positive cells in the platelet-rich fibrin 1 group were significantly higher than those in the control group (P > 0.05) and there was no significant difference between platelet-rich fibrin 1 group and platelet-rich fibrin 2 group (P > 0.05).  Results indicate that platelet-rich fibrin can promote the proliferation of bone marrow mesenchymal stem cells in a dose-dependent manner, and platelet-rich fibrin can induce bone marrow mesenchymal stem cells to differentiate into a high proportion of Schwann cells. The platelet-rich fibrin induction is superior to conventional chemical inducation.

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